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Mary K. Boudreaux, DVM, PhD, Professor, Department of Pathobiology

Dr. Mary K. Boudreaux, Professor in the Department of Pathobiology, earned the DVM degree in 1979 at Louisiana State University.  She practiced veterinary medicine in California before beginning a clinical pathology residency program under Dr. Julia Blue at Cornell in 1981. She developed an interest in hemostasis and platelet pathophysiology and in 1986 earned a PhD degree in a program jointly sponsored by Cornell University and the Wadsworth Center for Laboratories and Research in Albany. Her co-directors for the research which evaluated regulatory control of cAMP phosphodiesterase in canine platelets were Dr. David O. Slauson, Dr. James Catalfamo, and Dr. W. J. Dodds. She joined the faculty at Auburn in 1986 and has developed research interests involving platelets.

334-844-2692
boudrmk@auburn.edu

New Publication Available for Students

Hemostasis: Lecture Notes

About the Author: 

Dr. Boudreaux has been teaching veterinary students about hemostasis for over 25 years. In an effort to provide veterinary students with concise, up-to-date, easy to understand information on hemostasis in domestic animals she developed her own hemostasis handout for Auburn veterinary students in 1986. Her research efforts in this area combined with the efforts of others has allowed continual updating of information each year. This document represents over 25 years of commitment to the teaching of hemostasis to veterinary students with an emphasis on normal hemostasis, acquired and inherited disorders of hemostasis, and methods of identification of hemostatic disorders in domestic animals.

About the Booklet: 

The aim of the booklet is to educate veterinary students in a Clinical Pathology course setting about the various aspects of hemostasis in an easy to understand and concise format. A summary of the components of normal hemostasis is provided followed by sections on platelets, coagulation, and fibrinolysis. The platelet and coagulation sections provide brief descriptions of acquired and inherited disorders that have been described in animals along with appropriate methods of sample collection, available diagnostic assays, and interpretation of test results. Diagnostic assays presented include biochemical as well as molecular methods when available. Tables near the end of the document assist with summarizing the information in a concise format. A study guide is provided to help students focus on learning specific aspects of hemostasis. Sample cases are also provided to assist the students with applying the information learned in a case-based, clinical-type format. References are included for students who wish to obtain more information on a particular topic.

Research Interests

Evaluating the role of platelets in disease pathogenesis and identification and characterization of congenital platelet disorders.           

My research interests include evaluating the role of platelets in disease pathogenesis and identification and characterization of congenital platelet disorders.  Our platelet laboratory can isolate mammalian  platelets from a variety of species for evaluation of platelet reactivity.  We have developed a monoclonal antibody (CAP1) that detects a receptor-induced binding site (RIBS) on canine fibrinogen.  The antibody has been useful in detection of  platelet activation in dogs using flow cytometry and has also been useful  in the characterization of congenital platelet disorders. 

Recently we have begun using flow cytometry and CAP1 to identify and characterize platelet disorders in animals that cannot be taken to Auburn University or other platelet testing facility.  These studies require 5 mls of CPDA-1 blood obtained from the affected animal and a normal control sent to my laboratory within 24 hours of blood collection.  For more information on these studies and prior to submission of samples, please contact Dr. Mary K Boudreaux (boudrmk@auburn.edu). 

As part of our interest in congenital platelet disorders we determined the normal gene sequences encoding platelet glycoproteins IIb and IIIa in dogs and horses.  This work led to the discovery of the genetic basis for Glanzmann thrombasthenia (GT) in Great Pyrenees dogs and Otterhounds as well as the identification of 2 separate mutations causing GT in horses. 

During the late summer and fall of 2006 distinct mutations in the gene encoding calcium diacylglycerol guanine nucleotide exchange factor I (CalDAG-GEFI) were identified as causing thrombopathia in Basset hounds, Eskimo Spitz, Landseers of European Continental Type (ECT), and Simmental cattle.  

During the summer of 2007 a mutation in the gene encoding beta1-tubulin was found to correlate with the macrothrombocytopenia described in Cavalier King Charles Spaniels.  Since that time the identical mutation has been identified in several other breeds including Chihuahua, Labrador retriever, Poodle, English Toy Spaniel, Shih Tzu, Labradoodle, Maltese, Jack Russell, and Havanese. 

Recently a distinct mutation in the gene encoding beta1-tubulin has been identified in Norfolk and Cairn Terriers. In 2009 a mutation in the gene encoding Kindlin-3, a signal transduction protein important in integrin activation in platelets and leukocytes, was identified in a German Shepherd dog.  The affected dog experienced life-long bleeding, high white cell counts, and susceptibility to infections.  This syndrome is referred to as LAD-I Variant or LAD-III.  Also in 2009 a mutation was identified in a gene encoding a platelet activating receptor in a Greater Swiss Mountain Dog.  This dog experienced prolonged bleeding following a routine spay.   

Information on testing for GT in Great Pyrenees or Otterhounds, for CalDAG-GEFI thrombopathias in  Basset hounds, Eskimo Spitz, or Landseers-ECT, for congenital macrothrombocytopenia in Cavalier King Charles Spaniels and other breeds, for LAD-I Variant in German Shepherd dogs, and for a platelet disorder in Greater Swiss Mountain Dogs can be found below:

Submission Forms

Selected Publications

  • Boudreaux MK:  Inherited Platelet Disorders.  Invited State of the Art Review.  Journal of Veterinary Emergency and Critical Care, 22(1):1-12, 2012.   Guest Editor-In-Chief for this Special Edition on Platelets. 
  • Boudreaux MK:  Unraveling the wonders of platelets.  Invited Guest Editorial.  Journal of Veterinary Emergency and Critical Care, 22(1):2012.
  • Christopherson PW, Spangler EA, Boudreaux MK. Evaluation and Clinical Application of Platelet Function Testing in Small Animal Practice. Veterinary Clinics of North America Small Animal Practice.  42(1):173-188,  2012.   Invited Review
  • Puetz J, Boudreaux MK.  Evaluation of the gene encoding calcium and diacylglycerol regulated guanine nucleotide exchange factor I (CalDAG-GEFI) in human patients with congenital qualitative platelet disorders.  Platelets  Epub ahead of print, August 5, 2011.
  • Fang J, Jensen E, Boudreaux M, Du L, Hawkins T, Koukouritaki S, Cometta K, Wilcox D.  Platelet gene therapy; improved hemostatic function for integrin αIIbβ3 deficient dogs.  Proc Natl Acad Sci U S A.  108(23):9583-8, 2011.
  • Boudreaux MK, Martin M.  P2Y12 receptor gene mutation associated with postoperative hemorrhage in a Greater Swiss Mountain dog.  Vet Clin Pathol   40(2):202-6, 2011.
  • Boudreaux MK, Osborne C, Herre A, Ramos Rivera E, Spangler E. Unique structure of the M loop region of beta1-tubulin may contribute to size variability of platelets in the Family Felidae.  Vet Clin Pathol  39(4):417-423, 2010.
  • Boudreaux MK, Wardrop KJ, Kiklevich V, Felsburg P, Snekvik K:  A mutation in the canine kindlin-3 gene associated with increased bleeding risk and susceptibility to infections. Thromb  Haemost 103(2):475-477, 2010.
  • Davis B, Toivio-Kinnucan M, Schuller S, Boudreaux MK:  Mutation in beta1-tubulin correlates with macrothrombocytopenia in Cavalier King Charles Spaniels. J Vet Int Med  22:540-545, 2008.
  • Boudreaux MK, Schmutz S, French PS: Calcium diacylglycerol guanine nucleotide exchange factor I (CalDAG-GEFI) gene mutations in a thrombopathic Simmental calf. Vet Pathol 44(6):932-935, 2007.
  • Boudreaux MK, Catalfamo JL, Klok M:  Calcium Diacylglycerol Guanine Nucleotide Exchange Factor I gene mutations associated with loss of function in canine platelets.  Translational Research  150:81-92, 2007.
  • Christopherson PW, van Santen VL, Livesey L, Boudreaux MK:  A 10-base-pair deletion in the gene encoding platelet glycoprotein IIb associated with Glanzmann Thrombasthenia in a horse.    J Vet Int Med  21(1):196-198, 2007.
  • Christopherson PW, Insalaco TA, van Santen VL, Livesey L, Bourne C, Boudreaux MK:  Characterization of the cDNA encoding alpha IIb and beta 3 in normal horses and two horses with Glanzmann thrombasthenia.  Vet Pathol, 43:78-82, 2006.
  • Goree M, Catalfamo JL, Aber S, Boudreaux MK:  Characterization of the mutations causing Hemophilia B in two domestic cats.  J Vet Intern Med, 19:200-204, 2005. 
  • Livesey L, Christopherson P, Hammond A, Perkins J, Toivio-Kinnucan M, Insalaco, Boudreaux MK:  Platelet dysfunction (Glanzmann’s Thrombasthenia) in horses.  J Vet Int Med, 19:917-919, 2005.
  • Brdecka D, Adin C,  Boudreaux MK, Crawford C, Randall S:  Successful
  • ovariectomy in a dog with Glanzmann Thrombasthenia.  JAVMA, 224(11):1-3, 2004.
  • Niemeyer GP, Boudreaux MK, Goodman-Martin SA, Monroe CM, Wilcox DA, Lothrop CD Jr:  Correction of a large animal model of type I Glanzmann’s thrombasthenia by nonmyeloblative bone marrow transplantation.  Experimental Hematology  31:1357-1362, 2003.
  • Boudreaux MK:  Platelets – Past, Present, and Future.  Vet Clin Pathol  30(3):103, 2001.  Guest Editorial.
  • Lipscomb DL, Bourne C, Boudreaux MK:  Nucleotide sequence of the canine alpha IIb gene from platelet-derived cDNA.  Am J Vet Res 62(9):1486-1492, 2001.
  • Boudreaux MK, Catalfamo JL:  The molecular basis for Glanzmann’s thrombasthenia in Otterhounds.  Am J Vet Res  62(11):1797-1804, 2001.
  • Boudreaux MK, Lipscomb DL:  Clinical, Biochemical, and Molecular Aspects of Glanzmann’s Thrombasthenia in humans and dogs.  Vet Pathol  38(3):249-260, 2001.
  • Lipscomb DL, Bourne C, Boudreaux MK:  DNA sequence of the canine platelet β3 gene from cDNA:  Comparison of canine and mouse β3 to segments that encode alloantigenic sites and functional domains of β3 in human beings.  J Lab Clin Med  134:313-321, 1999.
  • Boudreaux MK, Lipscomb DL, Catalfamo JL:  Novel mutations within calcium binding domains of canine platelet GPIIb cause Type I Glanzmann’s thrombasthenia.  Blood, 94(10) Supplement 1 (Part 2): 77b, 1999 (abstract).
  • Lipscomb D, Boudreaux M, Bourne C:  Comparison of the alpha IIb nucleotide sequence from platelet cDNA of thrombasthenic and normal dogs.  Vet Pathol  36(5):482, 1999 (abstract).
  •  Boudreaux MK, Catalfamo JL:  Evaluation of the genes encoding for GP complex IIb-IIIa in Otterhounds with thrombasthenic thrombopathia.  Vet Pathol 36(5):483, 1999 (abstract).
  • Boudreaux MK, Kvam K, Dillon AR, Bourne C, Scott M, Schwartz KA, Toivio-Kinnucan M: Type I Glanzmann's thrombasthenia in a Great Pyrenees dog. Vet Pathol 33:503-511, 1996.
  • Boudreaux MK, Panangala VS, Bourne C: A platelet activation-specific monoclonal antibody that recognizes a receptor-induced binding site on canine fibrinogen. Vet Pathol 33:419-427, 1996.
  • Boudreaux MK: Platelets and Coagulation: An update: Vet Clin North America Small Animal  Practice  26(5):1065-1087, 1996.
  • Boudreaux MK, Crager C, Dillon AR, Stanz K, Toivio-Kinnucan M: Identification of an intrinsic platelet function defect in Spitz dogs. J Vet Int Med 8:93-98, 1994.
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